Cre Recombinase adenovirus
|Name:|| Ad-Cre-GFP |
|Cat. No. || 1700|
|Category:|| Pre-packaged Ready-to-use Adenovirus|
|Gene Name:|| Cre|
|Gene Alias:|| |
|Viral Backbone:|| human Adenovirus Type5 (dE1/E3)|
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.
This adenovirus expresses both Cre recombinase and GFP as marker. Cre and GFP are driven by 2 separate promoters.
Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).
Titer: 1x1010 PFU/ml
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
Availability: In stock, same day shipping available for orders placed before 1PM.
Published reference for this product:
Covered by US patent no. 6,974,694 and sold under license from AdVec Inc. |
Available for sale to academic, government and non-profit research institutes. All other purchasers require a commercial license for all fields including research use.
Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression. J. Virol. 69:4600-4606, 1995.
High frequency recombination between LoxP sites in human chromosomes mediated by an adenovirus vector expressing Cre recombinase. Somatic Cell and Molec. Genet. 21: 429-441, 1995.
The yeast recombinase FLP functions effectively in human cells for construction of adenovirus vectors. BioTechniques 29: 524-528, 2000.