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Cre Recombinase adenovirus


Name: Ad-Cre-GFP
Cat. No. 1700
Category: Pre-packaged Ready-to-use Adenovirus
Gene Name: Cre
Gene Alias:
Viral Backbone: human Adenovirus Type5 (dE1/E3)

Description:

Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.

This adenovirus expresses both Cre recombinase and GFP as marker. Cre and GFP are driven by 2 separate promoters.

Ad-Cre Video
Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).

Titer: 1x1010 PFU/ml

Volume: 200ul

Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol

Availability: In stock, same day shipping available for orders placed before 1PM.

Published reference for this product:

Catalog No. Tag Promoter Species Price **
1700 eGFP CMV $475.00


Covered by US patent no. 6,974,694 and sold under license from AdVec Inc.

Available for sale to academic, government and non-profit research institutes. All other purchasers require a commercial license for all fields including research use.

References:

Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression. J. Virol. 69:4600-4606, 1995.

High frequency recombination between LoxP sites in human chromosomes mediated by an adenovirus vector expressing Cre recombinase. Somatic Cell and Molec. Genet. 21: 429-441, 1995.

The yeast recombinase FLP functions effectively in human cells for construction of adenovirus vectors. BioTechniques 29: 524-528, 2000.

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