mouse TOP3A shRNA silencing AAV
Category: AAV > shRNA-Silencing
Cat. No: shAAV-281173
Availability:
6-8 weeks
Name:
AAV-m-TOP3A-shRNA
This is an AAV expressing shRNA for silencing of Mouse Top3a.
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Product Info
- Knockdown
- shRNA screening service available, please inquire about this service or other options.
Viral Details
- Viral Backbone
- Recombinant AAV
- AAV-ITR
- AAV2
- AAV Serotype
- Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
- Promoter
- U6
- Reporter
- eGFP (default), optional CFP, YFP, RFP, mCherry
- Storage Buffer
- PBS/5% Glycerol
Gene Details
- Species
- Mouse
- Gene Symbol
- Top3a
- Gene Name
- topoisomerase (DNA) III alpha
- Gene ID
- 21975
- ORF Size
- 3012 bp
- RefSeq#
- NM_009410
- Gene Description
- Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.
- UniGene ID
- Mm.477819
Related Products
Product | Cat No. |
---|---|
AAV-h-TOP3A | AAV-226206 |
AAV-m-TOP3A | AAV-281173 |
Ad-h-TOP3A | ADV-226206 |
Ad-m-TOP3A | ADV-281173 |
AAV-h-TOP3A-shRNA | shAAV-226206 |
Ad-h-TOP3A-shRNA | shADV-226206 |
Ad-m-TOP3A-shRNA | shADV-281173 |
Control Products
Product | Cat No. |
---|---|
AAV1-GFP-U6-shRNA | 7040 |
AAV2-GFP-U6-shRNA | 7041 |
AAV5-GFP-U6-shRNA | 7042 |
AAV6-GFP-U6-shRNA | 7043 |
AAV9-GFP-U6-shRNA | 7045 |
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