human SARS2 shRNA silencing AAV

Name
AAV-h-SARS2-shRNA

Cat No
shAAV-222261

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human SARS2.

shAAV-222261

AAV-h-SARS2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SARS2 (seryl-tRNA synthetase 2, mitochondrial). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

mtSerRS; SARS; SARSM; SerRS; SerRSmt; SERS; SYS

Description (Vector)

SARS2 encodes the mitochondrial seryl-tRNA synthethase precursor, a member of the class II tRNA synthetase family. The mature enzyme catalyzes the ligation of Serine to tRNA(Ser) and participates in the biosynthesis of selenocysteinyl-tRNA(sec) in mitochondria. The enzyme contains an N-terminal tRNA binding domain and a core catalytic domain. It functions in a homodimeric form, which is stabilized by tRNA binding. SARS2 is regulated by a bidirectional promoter that also controls the expression of mitochondrial ribosomal protein S12. Both genes are within the critical interval for the autosomal dominant deafness locus DFNA4 and might be linked to this disease. Multiple transcript variants encoding different isoforms have been identified for this gene.

Gene ID

54938

Gene Name (Vector)

seryl-tRNA synthetase 2, mitochondrial

Gene Symbol

SARS2

HGNC ID

HGNC:17697

ORF Size (aa)

1557

ORF Size (bp)

1557 bp

RefSeq ID

NM_017827

RefSeq Synonyms

NM_017827, NM_001145901, BC042912,

Species

human

UniGene ID

Hs.709416

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.