human PYCARD shRNA silencing AAV

Name 
AAV-h-PYCARD-shRNA
Cat No 
shAAV-220439
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PYCARD.

shAAV-220439
AAV-h-PYCARD-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PYCARD (PYD and CARD domain containing). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ASC; CARD5; TMS; TMS-1; TMS1
Description (Vector)
PYCARD encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
29108
Gene Name (Vector)
PYD and CARD domain containing
Gene Symbol
PYCARD
HGNC ID
HGNC:16608
ORF Size (aa)
408
RefSeq ID
NM_013258
RefSeq Synonyms
NM_145182, NM_013258, BC004470,
Species
human
UniGene ID
Hs.499094

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.