AAV with ALB(1.9) promoter driven fLuc
Cat. No: VB1585
Availability:
Immediate
Name:
AAV-ALB(1.9)-fLuc
This AAV expresses fLuc driven by a liver ALB(1.9) promoter.
The ALB(1.9) is a synthetic promoter of ~1.9 Kb. It is based on the human Albumin promoter as well as several other regulatory elements. This synthetic promoter gives an extremely high level of long-term, liver-specific transgene expression that is 100-100 fold greater than that of the CMV promoter. For short-term (1-2 weeks post-injection into mouse liver), ALB(1.9) gives comparable expression relative to that of the CMV promoter. However, the expression level of the CMV promoter decreases 100-1000 fold from its peak level within 10-20 weeks of DNA administration whereas the ALB(1.9) promoter sustains its initial high level of expression for over 1 year. Compared to the regular ALB promoter, which is also commonly used for liver-specific transgene expression, the expression level from ALB(1.9) is about 10-100 fold greater.
Firefly luciferase (fLuc) is an oxidative enzyme used in bioluminescence and is distinct from a photoprotein. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by a light-sensitive apparatus such as a luminometer or modified optical microscope. Firefly luciferase is commonly used as a reporter to assess the transcriptional activity in cells.
The ALB(1.9) is a synthetic promoter of ~1.9 Kb. It is based on the human Albumin promoter as well as several other regulatory elements. This synthetic promoter gives an extremely high level of long-term, liver-specific transgene expression that is 100-100 fold greater than that of the CMV promoter. For short-term (1-2 weeks post-injection into mouse liver), ALB(1.9) gives comparable expression relative to that of the CMV promoter. However, the expression level of the CMV promoter decreases 100-1000 fold from its peak level within 10-20 weeks of DNA administration whereas the ALB(1.9) promoter sustains its initial high level of expression for over 1 year. Compared to the regular ALB promoter, which is also commonly used for liver-specific transgene expression, the expression level from ALB(1.9) is about 10-100 fold greater.
Firefly luciferase (fLuc) is an oxidative enzyme used in bioluminescence and is distinct from a photoprotein. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by a light-sensitive apparatus such as a luminometer or modified optical microscope. Firefly luciferase is commonly used as a reporter to assess the transcriptional activity in cells.
Request a Quote
Please enter your email address and we'll be in touch with more information:
Viral Details
- Viral Backbone
- Recombinant AAV
- AAV-ITR
- AAV2
- AAV Serotype
- Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
- Promoter
- ALB(1.9) (liver)
- Storage Buffer
- PBS/5% Glycerol
- Volume
- 200ul
- Titer
- 1x10^13 GC/ml
Vector Biolabs
293 Great Valley Parkway
Malvern, PA 19355
Email: info@vectorbiolabs.com
Phone: +1 484-325-5100
Toll-free (US Only): 877-BIO-LABS
Fax: +1 215-525-1112
Privacy Policy