AAV with aMHC promoter driven fLuc
This AAV expresses fLuc driven by a cardiac aMHC promoter.
αMHC(0.4) promoter is derived from the murine α-MHC gene (alpha-myosin heavy chain) and drives cardiac-specific expression. The length of this promoter is 0.4 Kb. Based on studies in primary cardiomyocytes, this aMHC(0.4) promoter gives an expression level that is comparable to that of a CMV promoter.
Firefly luciferase (fLuc) is an oxidative enzyme used in bioluminescence and is distinct from a photoprotein. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by a light-sensitive apparatus such as a luminometer or modified optical microscope. Firefly luciferase is commonly used as a reporter to assess the transcriptional activity in cells.
Ready-to-use AAV expressing fLuc driven by a cardiac aMHC promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.