mouse USP5 shRNA silencing AAV

Product Info

Knockdown

>90% knockdown of mRNA in cultured cells

Viral Details

Viral Backbone

Recombinant AAV

AAV-ITR

AAV2

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

Promoter

U6

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Gene Details

Species

Mouse

Gene Symbol

Usp5

Gene Name

ubiquitin specific peptidase 5 (isopeptidase T)

Gene ID

22225

Gene Synonyms

AA407472; ISOT; ISOT-1; Ucht

ORF Size

2577 bp

RefSeq#

NM_013700

Gene Description

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

UniGene ID

Mm.3571