AAV with MBP promoter driven FLPo
Cat. No: VB5167
Availability:
2-3 weeks
Name:
AAV-MBP-FLPo-WPRE
This AAV expresses FLPo driven by an olgiodendricyte MBP promoter.
The myelin basic proteins (MBPs) are a family of polypeptides that are predominantly expressed in the nervous system, where they play a major role in myelination. This 1.3 Kb MBP promoter drives reporter gene expression mainly in oligodendrocytes.
FLPo is a codon-optimized version of FLPe that greatly increases the protein expression and the FRT recombination efficiency in mouse cells. FLP is a site-specific recombinase (SSR) from the _ integrase family which recognizes distinct 34 bp FRT sites. Like Cre/LoxP system, the FLP/FRT system has been widely used for gene expression and generating conditional knockout mice, mediated by the FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermal instability of the FLP protein. Subsequent screening for thermostable mutants resulted in the identification of FLPe which has a 4-fold higher recombination efficiency at 37oC than original FLP. However, the recombination efficiency of FLPe in cells remains very low relative to other recombinases because of its non-mammalian origin. FLPo is engineered by codon optimization of FLPe, and gives much higher expression and activity than FLPe.
The myelin basic proteins (MBPs) are a family of polypeptides that are predominantly expressed in the nervous system, where they play a major role in myelination. This 1.3 Kb MBP promoter drives reporter gene expression mainly in oligodendrocytes.
FLPo is a codon-optimized version of FLPe that greatly increases the protein expression and the FRT recombination efficiency in mouse cells. FLP is a site-specific recombinase (SSR) from the _ integrase family which recognizes distinct 34 bp FRT sites. Like Cre/LoxP system, the FLP/FRT system has been widely used for gene expression and generating conditional knockout mice, mediated by the FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermal instability of the FLP protein. Subsequent screening for thermostable mutants resulted in the identification of FLPe which has a 4-fold higher recombination efficiency at 37oC than original FLP. However, the recombination efficiency of FLPe in cells remains very low relative to other recombinases because of its non-mammalian origin. FLPo is engineered by codon optimization of FLPe, and gives much higher expression and activity than FLPe.
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Viral Details
- Viral Backbone
- Recombinant AAV
- AAV-ITR
- AAV2
- AAV Serotype
- Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
- Promoter
- MBP (olgiodendricyte)
- Storage Buffer
- PBS/5% Glycerol
- Volume
- 200ul
- Titer
- 1x10^13 GC/ml
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