Enh358MCK promoter driven Cas9/CRISPR AAV
The Cas9 from Streptococcus pyogenes SF370 (SpCas9) is too big to be packaged into AAV for most promoters, and there's no extra room for including a sgRNA in the same payload with spCas9 in AAV. A small Cas9 from Staphylococcus aureus subsp. aureus (SaCas9) with high efficiency for cleaving mammalian endogenous DNA is the ideal solution to package into AAV along with sgRNA for effective gene modification in vivo.
This is an AAV-DJ (a synthetic serotype made from 8 wild type AAV including AAV2, 4, 5, 8, 9) expressing spCas9 nuclease under a muscle specific tMCK promoter. This AAV-Enh358MCK-saCas9 can be package into other AAV serotypes upon request. The Enh358MCK promoter is constructed by ligating the ~200bp enhancer with the 358 bp proximal promoter of the murine MCK gene.
We also offer custom service to package AAV-Enh358MCK-saCas9 with your sgRNA in the same AAV virus. Please contact us for detail.
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- Viral Backbone
- Recombinant AAV
- AAV Serotype
- Enh358MCK ()
- Storage Buffer
- PBS/5% Glycerol
- 50 ul
- 1x10^13 GC/ml
- Gene Symbol