Cre Inducible saCas9/CRISPR AAV
The main problem with Cas9 from Streptococcus pyogenes SF370 (SpCas9) is that this gene is too big to be packaged into AAV under most promoters, and there's no extra room for packaging a sgRNA along with the spCas9 in AAV. A small Cas9 from Staphylococcus aureus subsp. aureus (SaCas9) with high efficiency for cleaving mammalian endogenous DNA becomes the ideal solution to package into AAV for effective gene modification in vivo.
This is an AAV-DJ expressing a Cre-inducible saCas9 under a small CMV7 promoter. The saCas9 is inserted in reverse orientation relative to the 5' CMV7 promoter and is flanked by oppositely oriented loxP and lox2272 sites. This AAV-CMV-DIO-saCas9 can be package into other AAV serotypes upon request.
We also offer custom AAV production service to package AAV-CMV-DIO-saCas9 with a sgRNA in same AAV virus. Please contact us for detail.
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- Viral Backbone
- Recombinant AAV
- AAV Serotype
- CMV7 (ubiquitous)
- Storage Buffer
- PBS/5% Glycerol
- 50 ul
- 1x10^13 GC/ml
- Gene Symbol
This product is referenced in the following publications: