CamKII driven saCas9/CRISPR AAV AAV
The main problem with Cas9 from Streptococcus pyogenes SF370 (SpCas9) is that this gene is too big to be packaged into AAV under most promoters, and there's no extra room for packaging a sgRNA along with the spCas9 in AAV. A small Cas9 from Staphylococcus aureus subsp. aureus (SaCas9) with high efficiency for cleaving mammalian endogenous DNA becomes the ideal solution to package into AAV for effective gene modification in vivo.
This is an AAV-DJ (a synthetic serotype made from 8 wild type AAV including AAV2, 4, 5, 8, 9) expressing spCas9 nuclease under a neuronal specific CamKIIa promoter. The short CamKIIa promoter was derived from murine a-Calcium/calmodulin-dependent kinase II (CaMKII), a gene with expression restricted to excitatory neurons in the neocortex and hippocampus. This AAV-CamKIIa-saCas9 can be package into other AAV serotypes upon request.
We also offer custom AAV production service to package AAV-CamKII-saCas9 with your sgRNA in the same AAV virus. Please contact us for detail.
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- Viral Backbone
- Recombinant AAV
- AAV Serotype
- CamKIIa ()
- Storage Buffer
- PBS/5% Glycerol
- 50 ul
- 1x10^13 GC/ml
- Gene Symbol