human RAD17 shRNA silencing Adenovirus

Name
Ad-h-RAD17-shRNA

Cat No
shADV-220634

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human RAD17.

shADV-220634

Ad-h-RAD17-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human RAD17 (RAD17 checkpoint clamp loader component). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Vector Designer

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

CCYC; cell cycle checkpoint protein (RAD17); cell cycle checkpoint protein RAD17; HRAD17; R24L; RAD1 homolog; RAD17 homolog; Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog

Description (eCommerce)

Rad17 is a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75943].

Description (Vector)

RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Gene ID

5884

Gene Name (eCommerce)

RAD17 checkpoint clamp loader component

Gene Name (Vector)

RAD17 checkpoint clamp loader component

Gene Symbol

RAD17

HGNC ID

HGNC:9807

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2013

ORF Size (bp)

2013 bp

Protein Name (eCommerce)

Cell cycle checkpoint protein RAD17

RefSeq ID

NM_133338

RefSeq Synonyms

NM_133344, NM_133343, NM_133342, NM_133341, NM_133340, NM_133339, NM_133338, NM_002873, NM_001278622, BC032304,

Research Areas

Cancer,Cell Cycle,DNA Damage/Repair,DNA Replication,Fanconi anemia,Homologous Recombination,Phospho-specific,Replication,Signal Transduction

Research Areas (Faceted)

cancer,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.16184

UniProt ID (eCommerce)

O75943

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.