human GRIA2 shRNA silencing AAV

Name
AAV-h-GRIA2-shRNA

Cat No
shAAV-210506

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human GRIA2.

shAAV-210506

AAV-h-GRIA2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GRIA2 (glutamate ionotropic receptor AMPA type subunit 2). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

GluA2; GluR-K2; GLUR2; GLURB; HBGR2

Description (Vector)

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene.

Gene ID

2891

Gene Name (Vector)

glutamate ionotropic receptor AMPA type subunit 2

Gene Symbol

GRIA2

HGNC ID

HGNC:4572

ORF Size (aa)

2652

ORF Size (bp)

2652 bp

RefSeq ID

NM_000826

RefSeq Synonyms

NM_001083620, NM_001083619, NM_000826, BC010574,

Species

human

UniGene ID

Hs.32763

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.