AAV with ELA1 promoter driven mCherry

Name 
AAV-ELA1-mCherry-WPRE
Cat No 
VB5346
Availability
3 weeks
Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses mCherry driven by a pancreatic acinar ELA1 promoter.

Rat elastase 1 (Ela1; ELAI1; RATELAI1), also called chymotrypsin-like elastase family, member 1, is the major protease with elastin-degrading activity in the mammalian exocrine pancreas. The ELA1 promoter (0.3Kb) is comprised of the core promoter and enhancer, and directs transgene expression in pancreatic acinar cells, stomach, duodenum, and colon.

mCherry was derived from DsRed through extensive mutagenesis. It has an Excitation/Emission spectrum of 590nm/610nm. mCherry’s emission spectrum is similar to the following fluorescent markers: mRuby (Em=605nM), JRed (Em=610nM), mRFP1 (Em=607nM), and HcRed1.

VB5346
AAV-ELA1-mCherry-WPRE

Ready-to-use AAV expressing mCherry driven by a pancreatic acinar ELA1 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
ELA1 (pancreatic acinar)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.