AAV with mIP1 promoter driven roGFP1

Name 
AAV-mIP1-roGFP1-WPRE
Cat No 
VB5268
Availability
3 weeks
Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses roGFP1 driven by an islet mIP1 promoter.

The mouse insulin-I promoter, (m)INS1, mIP-I or mIP1, is a promoter fragment of about 1.2 kb in length from the murine preproinsulin-I gene. It drives transgene expression in beta- cells of the pancreas, in the research field of diabetes and obesity.

roGFP1 is a reduction-oxidation sensitive green fluorescent protein used as a biosensor. Two cysteines were introduced into the beta-barrel structure of GFP; the oxidation state, reduced dithiol or the oxidized disulfide, of the engineered thiols determines the fluorescent properties of the sensor. roGFPs (roGFP1 and roGFP2), have two fluorescent excitation maxima at about 400 and 490 nm, respectively and display rapid and reversible ratiometric changes in fluorescence in response to changes in redox potential in vitro and in vivo.

VB5268
AAV-mIP1-roGFP1-WPRE

Ready-to-use AAV expressing roGFP1 driven by an islet mIP1 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
mIP1 (islet)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.