AAV with EF1a promoter driven EYFP

Name
AAV-EF1a-EYFP-WPRE

Cat No
VB4812

Availability
Immediate

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses EYFP driven by an ubiquitous EF1a promoter.

The 1.2 Kb EF1α promoter is a commonly used, constitutive promoter derived from human elongation factor-1 alpha (EF1α). It is often used in conditions where the other constitutive promoters, such as CMV, have diminished activity or have been silenced.

YFP is the longest wavelength-emitting variants of Aequorea GFP. The enhanced version, EYFP, has become one of the brightest and most widely utilized of the fluorescent proteins with an Excitation/Emission spectrum centered around 514nm/527nm. Other fluorescent proteins with similar emission spectrums include Topaz, Venus, Citrine and mCitrine.

VB4812

AAV-EF1a-EYFP-WPRE

Ready-to-use AAV expressing EYFP driven by an ubiquitous EF1a promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

EF1a (ubiquitous)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.