AAV with ALB(1.0) promoter driven LacZ

Name
AAV-ALB(1.0)-LacZ

Cat No
VB1700

Availability
3 weeks

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses LacZ driven by a liver ALB(1.0) promoter.

The ALB(1.0) is a shorter version of the ALB(1.4) promoter with a total length of 1.0 Kb. In mouse liver cells, the transgene expression from this ALB(1.0) synthetic promoter is about 3-5 times lower than that of ALB(1.4) promoter but 10-20 fold greater than that of the CMV promoter 10-20 weeks after plasmid injection into mouse liver.

The bacterial enzyme -galactosidase (gene LacZ) is frequently used as a reporter gene. It can be easily located with LacZ staining using the artificial substrate X-gal, which turns blue when cleaved by -galactosidase (LacZ).

VB1700

AAV-ALB(1.0)-LacZ

Ready-to-use AAV expressing LacZ driven by a liver ALB(1.0) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

ALB(1.0) (liver)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.