AAV with aMHC promoter driven LacZ
This AAV expresses LacZ driven by a cardiac aMHC promoter.
αMHC(0.4) promoter is derived from the murine α-MHC gene (alpha-myosin heavy chain) and drives cardiac-specific expression. The length of this promoter is 0.4 Kb. Based on studies in primary cardiomyocytes, this aMHC(0.4) promoter gives an expression level that is comparable to that of a CMV promoter.
The bacterial enzyme -galactosidase (gene LacZ) is frequently used as a reporter gene. It can be easily located with LacZ staining using the artificial substrate X-gal, which turns blue when cleaved by -galactosidase (LacZ).
VB1022
AAV-aMHC-LacZ
Ready-to-use AAV expressing LacZ driven by a cardiac aMHC promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.