AAV with CMV7 promoter driven fLuc

Name 
AAV-CMV7-fLuc
Cat No 
VB1512
Availability
3 weeks
Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses fLuc driven by an ubiquitous CMV7 promoter.

The CMV7 promoter is about 0.35 kb in length and is a shortened version of the commonly used CMV promoter. CMV7 has a truncated version of the CMV enhancer. When tested in several cell lines, this promoter provides an expression level that is about 3-5 fold lower than that of the original 0.6Kb CMV promoter.

Firefly luciferase (fLuc) is an oxidative enzyme used in bioluminescence and is distinct from a photoprotein. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by a light-sensitive apparatus such as a luminometer or modified optical microscope. Firefly luciferase is commonly used as a reporter to assess the transcriptional activity in cells.

VB1512
AAV-CMV7-fLuc

Ready-to-use AAV expressing fLuc driven by an ubiquitous CMV7 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV7 (ubiquitous)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.