AAV with hRPE(1.5) promoter driven Mito-roGFP1

Name 
AAV-hRPE(1.5)-Mito-roGFP1
Cat No 
VB1251
Availability
3 weeks
Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses Mito-roGFP1 driven by a retina hRPE(1.5) promoter.

The RPE65 gene encodes a protein of the retinal pigment epithelium (RPE) with a key role in the cycling of retinoids. This 1.5Kb promoter gives RPE-specific expression, but the expression level is not as high as that of the shorter, 0.8Kb, promoter. This is probably due to a negative regulatory element(s) present in the 1.5Kb promoter.

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.

VB1251
AAV-hRPE(1.5)-Mito-roGFP1

Ready-to-use AAV expressing Mito-roGFP1 driven by a retina hRPE(1.5) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
hRPE(1.5) (retina)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.