AAV with mIP2 promoter driven Mito-roGFP1

Name
AAV-mIP2-Mito-roGFP1-WPRE

Cat No
VB5295

Availability
3 weeks

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses Mito-roGFP1 driven by an islet mIP2 promoter.

This promoter is based on the 1.1 Kb regulatory region of mouse pre-proinsulin II gene, also known as ins2, ins-2, ins-II, insulin-II, or Mody4. It is commonly used for beta cell-specific gene expression in the pancreatic islet. (m)INS2 promoter is also referred as the mIP2 or mIP2 promoter.

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.

VB5295

AAV-mIP2-Mito-roGFP1-WPRE

Ready-to-use AAV expressing Mito-roGFP1 driven by an islet mIP2 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

mIP2 (islet)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.