AAV with GFAP(2.2) promoter driven Mito-roGFP1

Name
AAV-GFAP(2.2)-Mito-roGFP1

Cat No
VB1179

Availability
3 weeks

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses Mito-roGFP1 driven by an astrocyte GFAP(2.2) promoter.

The GFAP(2.2) promoter is based on human glial fibrillary acidic protein gene (GFAP), which is the major intermediate filament protein in astrocytes. It has been shown that approximately 2 kb of 5′-flanking DNA of the human GFAP gene is sufficient to direct transgene expression to astrocytes and confer developmental/injury-induced regulation.

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.

VB1179

AAV-GFAP(2.2)-Mito-roGFP1

Ready-to-use AAV expressing Mito-roGFP1 driven by an astrocyte GFAP(2.2) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

GFAP(2.2) (astrocyte)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.