mouse CDC42BPB shRNA silencing Adenovirus

Name 
Ad-m-CDC42BPB-shRNA
Cat No 
shADV-255097
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Cdc42bpb.

shADV-255097
Ad-m-CDC42BPB-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Cdc42bpb (CDC42 binding protein kinase beta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
MRCKb
Description (Vector)
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A.
Gene ID
217866
Gene Name (Vector)
CDC42 binding protein kinase beta
Gene Symbol
Cdc42bpb
ORF Size (aa)
5142
ORF Size (bp)
5142 bp
RefSeq ID
NM_183016
RefSeq Synonyms
NM_183016, BC138037, BC138035,
Species
mouse
UniGene ID
Mm.27397

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.