human SQSTM1 shRNA silencing Adenovirus

Name 
Ad-h-SQSTM1-shRNA
Cat No 
shADV-224302
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human SQSTM1.

shADV-224302
Ad-h-SQSTM1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SQSTM1 (sequestosome 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A170; DMRV; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; FTDALS3; NADGP; OSIL; oxidative stress induced like; p60; p62; p62B; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; sequestosome-1; ubiquitin-binding protein p62; ZIP3
Description (eCommerce)
Sequestosome-1 is an adapter protein that binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. Sequestosome-1 may play a role in titin/TTN downstream signaling in muscle cells, and may regulate signaling cascades through ubiquitination. Sequestosome-1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13501].
Description (Vector)
SQSTM1 encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Gene ID
8878
Gene Name (eCommerce)
sequestosome 1
Gene Name (Vector)
sequestosome 1
Gene Symbol
SQSTM1
HGNC ID
HGNC:11280
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1071
Protein Name (eCommerce)
Sequestosome-1
RefSeq ID
NM_003900
RefSeq Synonyms
NM_003900, NM_001142299, NM_001142298, BC019111, BC017222, BC003139, BC001874,
Research Areas
Apoptosis,Autophagy,BDNF Signaling,Cytokine Signaling,Immunology,Interleukin Signaling,Lysosome,Neurobiology,NF-kB Signaling,NGF Signaling,RANKL-RANK Signaling,Signal Transduction,TNF Signaling,Toll-like Receptor Signaling,Transcription Factor/Regulator,Transport,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cell_biology,immunology,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Sequestosome-1 is an adapter protein that binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. Sequestosome-1 may play a role in titin/TTN downstream signaling in muscle cells, and may regulate signaling cascades through ubiquitination. Sequestosome-1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13501].
UniGene ID
Hs.437277
UniProt ID (eCommerce)
Q13501

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.