mouse TOP3B shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Top3b.
shAAV-274819
AAV-m-TOP3B-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Top3b (topoisomerase (DNA) III beta). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
Gene ID
21976
Gene Name (Vector)
topoisomerase (DNA) III beta
Gene Symbol
Top3b
ORF Size (aa)
2589
ORF Size (bp)
2589 bp
RefSeq ID
NM_011624
RefSeq Synonyms
NM_011624, NM_001326576, BC031723,
Species
mouse
UniGene ID
Mm.326089
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.