mouse RASSF1 shRNA silencing AAV

Name
AAV-m-RASSF1-shRNA

Cat No
shAAV-270192

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Rassf1.

shAAV-270192

AAV-m-RASSF1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Rassf1 (Ras association (RalGDS/AF-6) domain family member 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

123F2; AA536941; AU044980; NORE2A; Rassf1A; Rassf1B; Rassf1C; RDA32; REH3P21

Description (Vector)

Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.

Gene ID

56289

Gene Name (Vector)

Ras association (RalGDS/AF-6) domain family member 1

Gene Symbol

Rassf1

ORF Size (aa)

813

RefSeq ID

NM_019713

RefSeq Synonyms

NM_019713, NM_001243748, BC002173,

Species

mouse

UniGene ID

Mm.12091

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.