mouse MAP4K2 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Map4k2.
shAAV-264202
AAV-m-MAP4K2-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Map4k2 (mitogen-activated protein kinase kinase kinase kinase 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AI385662; BL44; GCK; Rab8ip
Description (Vector)
Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion.
Gene ID
26412
Gene Name (Vector)
mitogen-activated protein kinase kinase kinase kinase 2
Gene Symbol
Map4k2
ORF Size (aa)
2334
ORF Size (bp)
2334 bp
RefSeq ID
NM_009006
RefSeq Synonyms
NM_009006, NM_001291787, BC116287, BC116286,
Species
mouse
UniGene ID
Mm.25860
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.