mouse EGLN2 shRNA silencing AAV

Name
AAV-m-EGLN2-shRNA

Cat No
shAAV-258139

Availability
Immediate

This is an AAV expressing shRNA for silencing of Mouse Egln2.

shAAV-258139

AAV-m-EGLN2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Egln2 (egl-9 family hypoxia-inducible factor 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

0610011A13Rik; C85656; Hif-p4h-1; Ier4; Phd1; SM-20

Description (Vector)

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death.

Gene ID

112406

Gene Name (Vector)

egl-9 family hypoxia-inducible factor 2

Gene Symbol

Egln2

ORF Size (aa)

1260

ORF Size (bp)

1260 bp

RefSeq ID

NM_053208

RefSeq Synonyms

NM_053208, BC086764, BC051436, BC023299,

Species

mouse

UniGene ID

Mm.29978

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.