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human VPS36 shRNA silencing AAV

Name 
AAV-h-VPS36-shRNA
Cat No 
shAAV-227532
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human VPS36.

shAAV-227532
AAV-h-VPS36-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human VPS36 (vacuolar protein sorting 36 homolog). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
C13orf9; CGI-145; EAP45
Description (Vector)
VPS36, VPS22 (SNF8; MIM 610904), and VPS25 (MIM 610907) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS36, VPS22, and VPS25 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 (PubMed 15755741); Kamura et al., 2001 (PubMed 11278625)).(supplied by OMIM, Mar 2008)
Gene ID
51028
Gene Name (Vector)
vacuolar protein sorting 36 homolog
Gene Symbol
VPS36
HGNC ID
HGNC:20312
ORF Size (aa)
1161
RefSeq ID
NM_016075
RefSeq Synonyms
NM_016075, NM_001282169, NM_001282168, BC050439, BC037279,
Species
human
UniGene ID
Hs.109520

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.