human VPS35 shRNA silencing AAV

Name 
AAV-h-VPS35-shRNA
Cat No 
shAAV-227529
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human VPS35.

shAAV-227529
AAV-h-VPS35-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human VPS35 (VPS35, retromer complex component). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
hVPS35; maternal-embryonic 3; MEM3; PARK17; vacuolar protein sorting 35 homolog; vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35
Description (eCommerce)
Vacuolar protein sorting-associated protein 35 (VPS35) belongs to a group of vacuolar protein sorting (VPS) genes. VPS35 is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network [taken from NCBI Entrez Gene (Gene ID: 55737)].
Description (Vector)
VPS35 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
Gene ID
55737
Gene Name (eCommerce)
VPS35, retromer complex component
Gene Name (Vector)
VPS35, retromer complex component
Gene Symbol
VPS35
HGNC ID
HGNC:13487
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2391
ORF Size (bp)
2391 bp
Protein Name (eCommerce)
Vacuolar protein sorting-associated protein 35
RefSeq ID
NM_018206
RefSeq Synonyms
NM_018206, BC093036, BC010362, BC002414,
Research Areas
Apoptosis,Golgi Apparatus,Neurobiology,Neurodegeneration,Parkinson's Disease,Signal Transduction,Transport,Wnt Signaling
Research Areas (Faceted)
cell_biology,neurobiology,signal_transduction
Species
human
Target Sentence
Vacuolar protein sorting-associated protein 35 (VPS35) belongs to a group of vacuolar protein sorting (VPS) genes. VPS35 is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network [taken from NCBI Entrez Gene (Gene ID: 55737)].
UniGene ID
Hs.454528
UniProt ID (eCommerce)
Q96QK1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.