human UBE2A shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human UBE2A.
shAAV-226987
AAV-h-UBE2A-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human UBE2A (ubiquitin conjugating enzyme E2 A). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
E2 ubiquitin-conjugating enzyme A; HHR6A; HR6A; MRXS30; MRXSN; RAD6 homolog A; RAD6A; UBC2; ubiquitin carrier protein A; ubiquitin-conjugating enzyme E2 A; ubiquitin-conjugating enzyme E2A; ubiquitin-protein ligase A
Description (eCommerce)
Rad 6, also known as Ubiquitin conjugating enzyme E2A, is an E2 enzyme of the three step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ubiquitination provides an important post-translational means to regulate protein abundance and activity. Rad6 has been demonstrated to be important to DNA damage repair. Mutations in Rad6 cause an X-linked mental retardation syndrome.
Description (Vector)
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
Gene ID
7319
Gene Name (eCommerce)
ubiquitin conjugating enzyme E2 A
Gene Name (Vector)
ubiquitin conjugating enzyme E2 A
Gene Symbol
UBE2A
HGNC ID
HGNC:12472
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
459
Protein Name (eCommerce)
Ubiquitin-conjugating enzyme E2 A
RefSeq ID
NM_003336
RefSeq Synonyms
NM_181777, NM_181762, NM_003336, NM_001282161, BC010175,
Research Areas
Antigen Processing and Presentation,Cancer,Cardiology,Cardiovascular,Complexome,DNA Damage/Repair,Histone Modification,Hypoxia,Immunology,Neurobiology,Proteasome,RNA Processing,Ubiquitination
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,genetics,immunology,neurobiology,transcription_translation
Species
human
Target Sentence
Rad 6, also known as Ubiquitin conjugating enzyme E2A, is an E2 enzyme of the three step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ubiquitination provides an important post-translational means to regulate protein abundance and activity. Rad6 has been demonstrated to be important to DNA damage repair. Mutations in Rad6 cause an X-linked mental retardation syndrome.
UniGene ID
Hs.379466
UniProt ID (eCommerce)
P49459
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.