human RASSF1 shRNA silencing AAV

Name
AAV-h-RASSF1-shRNA

Cat No
shAAV-220797

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human RASSF1.

shAAV-220797

AAV-h-RASSF1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RASSF1 (Ras association domain family member 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

123F2; NORE2A; RASSF1A; RDA32; REH3P21

Description (Vector)

RASSF1 encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Gene ID

11186

Gene Name (Vector)

Ras association domain family member 1

Gene Symbol

RASSF1

HGNC ID

HGNC:9882

ORF Size (aa)

1023

ORF Size (bp)

1023 bp

RefSeq ID

NM_007182

RefSeq Synonyms

NM_170714, NM_170713, NM_170712, NM_007182, NM_001206957, BC143879, BC117153, BC110412,

Species

human

UniGene ID

Hs.476270

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.