human PRF1 shRNA silencing AAV

Name
AAV-h-PRF1-shRNA

Cat No
shAAV-219811

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human PRF1.

shAAV-219811

AAV-h-PRF1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRF1 (perforin 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

FLH2; HPLH2; P1; PFN1; PFP

Description (Vector)

PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein.

Gene ID

5551

Gene Name (Vector)

perforin 1

Gene Symbol

PRF1

HGNC ID

HGNC:9360

ORF Size (aa)

1668

ORF Size (bp)

1668 bp

RefSeq ID

NM_005041

RefSeq Synonyms

NM_005041, NM_001083116, BC063043, BC047695,

Species

human

UniGene ID

Hs.2200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.