human MAN1B1 shRNA silencing AAV

Name
AAV-h-MAN1B1-shRNA

Cat No
shAAV-214875

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human MAN1B1.

shAAV-214875

AAV-h-MAN1B1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MAN1B1 (mannosidase alpha class 1B member 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ERMAN1; ERManI; MANA-ER; MRT15

Description (Vector)

MAN1B1 encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11.

Gene ID

11253

Gene Name (Vector)

mannosidase alpha class 1B member 1

Gene Symbol

MAN1B1

HGNC ID

HGNC:6823

ORF Size (aa)

2100

ORF Size (bp)

2100 bp

RefSeq ID

NM_016219

RefSeq Synonyms

NM_016219, BC006079, BC002953,

Species

human

UniGene ID

Hs.279881

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.