human ETFA shRNA silencing AAV

Name 
AAV-h-ETFA-shRNA
Cat No 
shAAV-208133
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ETFA.

shAAV-208133
AAV-h-ETFA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ETFA (electron transfer flavoprotein alpha subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
EMA; GA2; MADD
Description (Vector)
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
2108
Gene Name (Vector)
electron transfer flavoprotein alpha subunit
Gene Symbol
ETFA
HGNC ID
HGNC:3481
ORF Size (aa)
1002
ORF Size (bp)
1002 bp
RefSeq ID
NM_000126
RefSeq Synonyms
NM_001127716, NM_000126, BC095457, BC015526,
Species
human
UniGene ID
Hs.39925

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.