mouse TUT1 Over-expression AAV

Name 
AAV-m-TUT1
Cat No 
AAV-275362
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Tut1.

AAV-275362
AAV-m-TUT1

Ready-to-use AAV expressing Mouse Tut1 (terminal uridylyl transferase 1, U6 snRNA-specific). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2700038E08Rik; PAPD2; Rbm21; star-PAP; TUTase6; U6-TUTase
Description (Vector)
Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
Gene ID
70044
Gene Name (Vector)
terminal uridylyl transferase 1, U6 snRNA-specific
Gene Symbol
Tut1
ORF Size (aa)
2610
ORF Size (bp)
2610 bp
RefSeq ID
NM_197993
RefSeq Synonyms
NM_197993, BC025499, BC023900,
Species
mouse
UniGene ID
Mm.165979

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.