mouse TUT1 shRNA silencing AAV

Name
AAV-m-TUT1-shRNA

Cat No
shAAV-275362

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Tut1.

shAAV-275362

AAV-m-TUT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Tut1 (terminal uridylyl transferase 1, U6 snRNA-specific). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2700038E08Rik; PAPD2; Rbm21; star-PAP; TUTase6; U6-TUTase

Description (Vector)

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.

Gene ID

70044

Gene Name (Vector)

terminal uridylyl transferase 1, U6 snRNA-specific

Gene Symbol

Tut1

ORF Size (aa)

2610

ORF Size (bp)

2610 bp

RefSeq ID

NM_197993

RefSeq Synonyms

NM_197993, BC025499, BC023900,

Species

mouse

UniGene ID

Mm.165979

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.