mouse EGLN2 Over-expression AAV

Name 
AAV-m-EGLN2
Cat No 
AAV-258139
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Egln2.

AAV-258139
AAV-m-EGLN2

Ready-to-use AAV expressing Mouse Egln2 (egl-9 family hypoxia-inducible factor 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
0610011A13Rik; C85656; Hif-p4h-1; Ier4; Phd1; SM-20
Description (Vector)
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death.
Gene ID
112406
Gene Name (Vector)
egl-9 family hypoxia-inducible factor 2
Gene Symbol
Egln2
ORF Size (aa)
1260
ORF Size (bp)
1260 bp
RefSeq ID
NM_053208
RefSeq Synonyms
NM_053208, BC086764, BC051436, BC023299,
Species
mouse
UniGene ID
Mm.29978

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.