AAV-CAG-GFP (AAV-DJ) AAV
The AAV-DJ is a synthetic serotype made from DNA family shuffling of 8 wild type serotypes of AAV, including AAV2, 4, 5, 8, 9, avian, bovine and goat AAV. AAV-DJ outperformed standard AAV serotype 1-8 in cultured cells.
The CAG promoter is a hybrid of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter. The CAG promoter is used to drive high levels of gene expression in mammalian expression vectors.
This AAV-DJ stock expresses eGFP under a control of CAG promoter.
7078
AAV/DJ-CAG-GFP
Ready-to-use AAV-CAG-GFP (AAV-DJ). AAV-DJ-eGFP AAVDJ-CAG-GFP AAV-CAG-GFP AAVDJ-CBA-GFP cat. 7078
Related Citations
- Increased Temperature Facilitates Adeno-Associated Virus Vector Transduction of Colorectal Cancer Cell Lines in a Manner Dependent on Heat Shock Protein Signature. A Bienkowska, etc, (2020), Biomed Research
- Mosaic Recombinant Adeno-associated Virus Vector rAAV/DJ/CAG for Targeted Gene Delivery to Melanoma Cells Metastasized to the Lung. M Czajka, etc, (2020), Anticancer Research
- Cellular selectivity of AAV serotypes for gene delivery in neurons and astrocytes by neonatal intracerebroventricular injection. Hammond SL, etc, (2018), PLoS
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.