AAV-CAG-GFP (AAV-DJ) AAV
The AAV-DJ is a synthetic serotype made from DNA family shuffling of 8 wild type serotypes of AAV, including AAV2, 4, 5, 8, 9, avian, bovine and goat AAV. AAV-DJ outperformed standard AAV serotype 1-8 in cultured cells.
The CAG promoter is a hybrid of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter. The CAG promoter is used to drive high levels of gene expression in mammalian expression vectors.
This AAV-DJ stock expresses eGFP under a control of CAG promoter.
7078
AAV/DJ-CAG-GFP
Ready-to-use AAV-CAG-GFP (AAV-DJ). AAV-DJ-eGFP AAVDJ-CAG-GFP AAV-CAG-GFP AAVDJ-CBA-GFP cat. 7078
Related Citations
- Increased Temperature Facilitates Adeno-Associated Virus Vector Transduction of Colorectal Cancer Cell Lines in a Manner Dependent on Heat Shock Protein Signature. A Bienkowska, etc, (2020), Biomed Research
- Mosaic Recombinant Adeno-associated Virus Vector rAAV/DJ/CAG for Targeted Gene Delivery to Melanoma Cells Metastasized to the Lung. M Czajka, etc, (2020), Anticancer Research
- Cellular selectivity of AAV serotypes for gene delivery in neurons and astrocytes by neonatal intracerebroventricular injection. Hammond SL, etc, (2018), PLoS
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.