FLAG tagged CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus

Name 
Ad(RGD)-mCherry-FLAG-hCas9
Cat No 
1907
Availability
Immediate

The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.

For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This RGD-fiber modified adenovirus expresses a codon-optimized Cas9 nuclease with N-terminal FLAG tag and a mCherry reporter. It can be used along with guided RNA for genome-editing in cells that regular Ad5 doesn’t infect well, including human or mouse macrophages, ES cells, T cells, synoviocytes, certain fibroblast and islet grafts etc.

1907
Ad(RGD)-mCherry-FLAG-hCas9

Ready-to-use FLAG tagged CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus. CRISPR Cas9 adenovirus Ad(RGD)-FLAG-Cas9 Ad(RGD)-mCherry-FLAG-Cas9 1907

$690.00
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Product Details

Promoter
CAG (ubiquitous)
Reporter
mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3) with RGD-fiber modification
Volume (µL)
200

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.