human BCL-xL shRNA Adenovirus

Name 
Ad-h-BCL-xL-shRNA
Cat No 
1808
Availability
Immediate

This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human BCL-xL gene. The shRNA expression is driven by an U6 promoter.

The knockdown of this human gene was validated by western blot in A549 cells.

1808
Ad-h-BCL-xL-shRNA

Ready-to-use human BCL-xL shRNA Adenovirus. human BCL-xL shRNA adenovirus gene knockdown1808

$995.00
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Product Details

Promoter
U6 ()
Reporter
eGFP
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
apoptosis regulator Bcl-X; BCL2L; Bcl2-L-1; bcl-2-like protein 1; BCLX; Bcl-X; BCL-XL/S; PPP1R52; protein phosphatase 1, regulatory subunit 52
Description (eCommerce)
Bcl-xL is part of the BCL-2 family of proteins that regulates apoptosis and cell cycle control. Bcl-xL is the protein product of the larger alternatively spliced isoform of Bcl-x. Bcl-xL functions similarly to BCL2 in promoting cell survival and inhibiting proliferation by promoting G0 arrest. Bcl-xL is located in the outer mitochondrial membrane and promotes cell survival by preventing mitochondrial release of cytochrome c and caspase activation in response to apoptotic signals.
Description (Vector)
BCL2L1 belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.
Gene ID
598
Gene Name (eCommerce)
BCL2 like 1
Gene Name (Vector)
BCL2 like 1
Gene Symbol
BCL2L1
HGNC ID
HGNC:992
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
702
ORF Size (bp)
702 bp
Protein Name (eCommerce)
Bcl-2-like protein 1
RefSeq ID
NM_001191
RefSeq Synonyms
NM_138578, NM_001322242, NM_001322240, NM_001322239, NM_001317921, NM_001317920, NM_001317919, NM_001191, BC019307,
Research Areas
Amyotrophic Lateral Sclerosis (ALS),Apoptosis,Autophagy,Cancer,CD40 Signaling,Cell Cycle,Cytoskeleton,DNA Damage/Repair,EPO Signaling,Host-Virus Interactions,Immunology,Innate Immunity,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,Leptin Signaling,Leukemia,Lung Cancer,Mitochondrion,Neurobiology,Neurodegeneration,Neurodevelopment,NFAT Signaling,NF-kB Signaling,Parkinson's Disease,PI3K/Akt Signaling,Signal Transduction,Spermatogenesis,Synapse,TNF Signaling,Transcriptional Misregulation in Cancer
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Bcl-xL is part of the BCL-2 family of proteins that regulates apoptosis and cell cycle control. Bcl-xL is the protein product of the larger alternatively spliced isoform of Bcl-x. Bcl-xL functions similarly to BCL2 in promoting cell survival and inhibiting proliferation by promoting G0 arrest. Bcl-xL is located in the outer mitochondrial membrane and promotes cell survival by preventing mitochondrial release of cytochrome c and caspase activation in response to apoptotic signals.
UniGene ID
Hs.516966
UniProt ID (eCommerce)
Q07817

Related Citations

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.