Tamoxifen inducible Cre (CreERT2-GFP) Adenovirus

Name 
Ad-GFP-CreERT2
Cat No 
1795
Availability
Immediate
Categories
Adenovirus, Over-Expression, Recombinase

A mutated form of estrogen ligand-binding domain (ERT2) that binds to synthetic antagonists (such as tamoxifen or its derivative 4-hydroxy-tamoxifen) but not to circulating estrogens was fused to the Cre recombinase (Cre) to create the CreERT2, which requires the presence of tamoxifen for Cre recombinase activity. Therefore it makes possible to induce the recombination in cells with tamoxifen.

This adenovirus expresses both CreERT2 and eGFP under the same CMV promoter, and the 2 genes are separated by a 2A polypeptide.

1795
Ad-GFP-CreERT2

Ready-to-use Tamoxifen inducible Cre (CreERT2-GFP) Adenovirus. CreERT2 Cre-ERT2 adenovirus 1795

$495.00
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Product Details

Promoter
CMV (ubiquitous)
Reporter
eGFP
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.