mouse POU domain, class 5, transcription factor 1 (POU5F1) Adenovirus

Name 
Ad-m-Oct4
Cat No 
1792
Availability
Immediate
Categories
Adenovirus, iPS stem cell factors, Over-Expression

Oct-3/4 is one of the family of octamer (“Oct”) transcription factors, and plays a crucial role in maintaining pluripotency. The absence of Oct-3/4 in Oct-3/4+ cells, such as blastomeres and embryonic stem cells, leads to spontaneous trophoblast differentiation, and presence of Oct-3/4 thus gives rise to the pluripotency and differentiation potential of embryonic stem cells. Various other genes in the “Oct” family, including Oct-3/4’s close relatives, Oct1 and Oct6, fail to elicit induction, thus demonstrating the exclusiveness of Oct-3/4 to the induction process.

Using adenovirus to deliver these transcription factors has 2 main advantages – it does not incorporate into the targeted host and therefore avoids the potential for insertional mutagenesis, and the expression is transient for a brief amount of time in order for effective reprogramming to take place.

This adenovirus expresses mouse Oct3/4, and it can be used in combination with other iPSC factor for the induction of iPSC reprogramming.

1792
Ad-m-Oct4

Ready-to-use mouse POU domain, class 5, transcription factor 1 (POU5F1) Adenovirus. Oct3 Oct4 adenovirus iPS 1792

$475.00
Request Volume Pricing or Customization

Product Details

Promoter
CMV (ubiquitous)
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
NF-A3; Oct3; Oct-3; Oct3/4; Oct-3/4; Oct4; Oct-4; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; octamer-binding transcription factor 4 alternative; Otf3; Otf-3; Otf3g; Otf3-rs7; Otf4; Otf-4; POU domain class 5 transcription factor 1 transcript variant 2 oct4b'; POU domain class 5 transcription factor 1 transcript variant 3 oct4b; POU domain, class 5, transcription factor 1
Description (eCommerce)
Oct-4 (Octamer-binding protein 4), also known as Oct 3/4, is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct-4 forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20263].
Description (Vector)
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Gene ID
18999
Gene Name (eCommerce)
POU domain, class 5, transcription factor 1
Gene Name (Vector)
POU domain, class 5, transcription factor 1
Gene Symbol
Pou5f1
NCBI Taxonomy ID (eCommerce)
10090.0
ORF Size (aa)
1059
Protein Name (eCommerce)
POU domain, class 5, transcription factor 1
RefSeq ID
NM_013633
RefSeq Synonyms
NM_013633, NM_001252452, BC068268,
Research Areas
Chromatin Remodeling
Research Areas (Faceted)
cell_biology,genetics
Species
mouse
Target Sentence
Oct-4 (Octamer-binding protein 4), also known as Oct 3/4, is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct-4 forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20263].
UniGene ID
Mm.17031
UniProt ID (eCommerce)
P20263

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.