human Krueppel-like factor 4 (KLF4) Adenovirus

Name 
Ad-h-KLF4
Cat No 
1787
Availability
Immediate
Categories
Adenovirus, iPS stem cell factors, Over-Expression

Klf4 of the Klf family of genes was initially identified by Yamanaka and confirmed by Jaenisch as a factor for the generation of mouse iPS cells and was demonstrated by Yamanaka as a factor for generation of human iPS cells. However, it was recently reported that Klf4 was unnecessary for generation of human iPS cells. Klf2 and Klf4 were found to be factors capable of generating iPS cells, and related genes Klf1 and Klf5 did as well, although with reduced efficiency.

Co-expression of Oct4, Sox2, KLF4 and c-Myc (Yamanaka set) in somatic cell will lead to iPSC reprogramming. Using adenovirus to deliver these transcription factors has 2 main advantages – it does not incorporate into the targeted host and therefore avoids the potential for insertional mutagenesis, and the expression is transient for a brief amount of time in order for effective reprogramming to take place.

This adenovirus expresses human KLF4, and it can be used in combination with other iPSC factor for the induction of iPSC reprogramming.

1787
Ad-h-KLF4

Ready-to-use human Krueppel-like factor 4 (KLF4) Adenovirus. KLF4 iPs adenovirus 1787

$475.00
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Product Details

Promoter
CMV (ubiquitous)
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
endothelial Kruppel-like zinc finger protein; epithelial zinc finger protein EZF; EZF; GKLF; gut Kruppel-like factor; gut-enriched krueppel-like factor; Krueppel-like factor 4; Kruppel-like factor 4 (gut)
Description (eCommerce)
KLF4 belongs to the Kruppel family of transcription factors. This zinc finger protein is required for normal development of the barrier function of skin. KLF4 is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. [taken from NCBI Entrez Gene (Gene ID: 9314)].
Gene ID
9314
Gene Name (eCommerce)
Kruppel like factor 4
Gene Name (Vector)
Kruppel like factor 4
Gene Symbol
KLF4
HGNC ID
HGNC:6348
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1413
ORF Size (bp)
1413 bp
Protein Name (eCommerce)
Krueppel-like factor 4
RefSeq ID
NM_004235
RefSeq Synonyms
NM_004235, NM_001314052, BC030811, BC029923,
Research Areas
Immunology,RNA Binding,Transcription Factor / Regulator,Zinc-finger
Research Areas (Faceted)
cell_biology,immunology,transcription_translation
Species
human
Target Sentence
KLF4 belongs to the Kruppel family of transcription factors. This zinc finger protein is required for normal development of the barrier function of skin. KLF4 is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. [taken from NCBI Entrez Gene (Gene ID: 9314)].
UniGene ID
Hs.376206
UniProt ID (eCommerce)
O43474

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.