Inhibitor of kappaB kinase beta Adenovirus

Name 
Ad-IKKb
Cat No 
1487
Availability
Immediate
Categories
Adenovirus, Over-Expression

The IkB kinases, IKK(alpha) (previously designated CHUK) and IKK(beta), are members of the helix-loop-helix, leucine zipper family of interacting proteins. IKK(alpha) specifically phosphorylates IkB(alpha) on the sites, serines 32 and 36, to trigger its degradation. The IKK complex appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK(alpha) is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation. The functional IKK complex contains three subunits, IKK(alpha), IKK(beta) and IKK(gamma) (also designated NEMO, for NFkB essential modulator), and each appear to make essential contributions to IkB phosphorylation. TANK binding kinase (TBK1), also designated T2K, is an IKK-related kinase that complexes with TRAF2 and TANK in the NF?B activation pathway. IKK-i is an IKK-related serine/threonine kinase that is expressed in immune cells and is inducible by LPS, TNF(alpha), IL-1 and IL-6. Overexpression of IKK-i results in IkB(alpha) phosphorylation and NFkB activation.

1487
Ad-IKKb

Ready-to-use Inhibitor of kappaB kinase beta Adenovirus. Ad-IKKb, Inhibitor of kappaB kinase beta, IKK2, IKK-2, IKK[b], IKK-beta, IKKb adenovirus 1487

$475.00
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Product Details

Promoter
CMV (ubiquitous)
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
AI132552; IKK-2; IKK-beta; IKK2; IKKbeta; IKK[b]
Description (Vector)
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
Gene ID
16150
Gene Name (Vector)
inhibitor of kappaB kinase beta
Gene Symbol
Ikbkb
ORF Size (aa)
2274
ORF Size (bp)
2274 bp
RefSeq ID
NM_010546
RefSeq Synonyms
NM_010546, NM_001159774, BC037723,
Species
mouse
UniGene ID
Mm.277886

Related Citations

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.