human c-Myc Adenovirus

Name 
Ad-h-Myc
Cat No 
1788
Availability
Immediate
Categories
Adenovirus, iPS stem cell factors, Over-Expression

The Myc family of genes are proto-oncogenes implicated in cancer. Yamanaka demonstrated that c-myc is a factor implicated in the generation of mouse and human iPS cells. However, Thomson and Yamanaka also reported that c-myc was unnecessary for generation of human iPS cells. Usage of the “myc” family of genes in induction of iPS cells is troubling for the eventuality of iPS cells as clinical therapies, as 25% of mice transplanted with c-myc-induced iPS cells developed lethal teratomas. N-myc and L-myc have been identified to induce instead of c-myc with similar efficiency.

Co-expression of Oct4, Sox2, KLF4 and c-Myc (Yamanaka set) in somatic cell will lead to iPSC reprogramming. Using adenovirus to deliver these transcription factors has 2 main advantages – it does not incorporate into the targeted host and therefore avoids the potential for insertional mutagenesis, and the expression is transient for a brief amount of time in order for effective reprogramming to take place.

This adenovirus expresses human c-Myc, and it can be used in combination with other iPSC factor for the induction of iPSC reprogramming.

1788
Ad-h-Myc

Ready-to-use human c-Myc Adenovirus. c-myc, myc, iPS adenovirus 1788

$475.00
Request Volume Pricing or Customization

Product Details

Promoter
CMV (ubiquitous)
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
avian myelocytomatosis viral oncogene homolog; bHLHe39; class E basic helix-loop-helix protein 39; c-Myc; MRTL; myc proto-oncogene protein; MYCC; myc-related translation/localization regulatory factor; proto-oncogene c-Myc; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
Description (eCommerce)
The Myc epitope tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized c-myc epitope represents amino acid residues 410-419, EQKLISEEDL, of human c-myc.
Description (Vector)
MYC is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
Gene ID
4609
Gene Name (eCommerce)
MYC proto-oncogene, bHLH transcription factor
Gene Name (Vector)
v-myc avian myelocytomatosis viral oncogene homolog
Gene Symbol
MYC
HGNC ID
HGNC:7553
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1365
ORF Size (bp)
1365 bp
Protein Name (eCommerce)
Myc proto-oncogene protein
RefSeq ID
NM_002467
RefSeq Synonyms
NM_002467, BC058901, BC000917, BC000141,
Research Areas
Apoptosis,B-cell Receptor Signaling,Bladder Cancer,Breast Cancer,Cancer,Cardiac Hypertrophy,Cardiology,Cardiovascular,CD40 Signaling,Cell Cycle,Chromatin Remodeling,Colorectal Cancer,DNA Damage/Repair,EGFR Signaling,Gene Expression,Hippo Signaling,Host-Virus Interactions,Immunology,Interleukin Signaling,JAK-STAT Signaling,Leukemia,LKB1 Signaling,Lung Cancer,MAPK Signaling,Metabolism/Metabolic Process,MicroRNAs in Cancer,Neurobiology,Neurodevelopment,Notch Signaling,PDGF Signaling,PI3K/Akt Signaling,Prolactin Signaling,Prostate Cancer,Signal Transduction,Telomeric Maintenance,TGF-beta Signaling,Transcription Factor/Regulator,Transcriptional Misregulation in Cancer,TSH Signaling,TSLP Signaling,Wnt Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,metabolism,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
The Myc epitope tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized c-myc epitope represents amino acid residues 410-419, EQKLISEEDL, of human c-myc.
UniGene ID
Hs.
UniProt ID (eCommerce)
P01106

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.