codon-improved Cre (iCre) and RFP Adenovirus

Name 
Ad-RFP-Cre
Cat No 
1774
Availability
Immediate
Categories
Adenovirus, Over-Expression, Recombinase

Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.

By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.

This adenovirus expresses both a codon improved Cre recombinase (iCre), and a RFP protein as fluorescent marker. Cre and RFP are driven by the same CMV promoter and separated by 2A peptides.

Ad-Cre Video
Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).

1774
Ad-RFP-Cre

Ready-to-use codon-improved Cre (iCre) and RFP Adenovirus. Ad-Cre-RFP Ad-RFP-Cre iCre RFP adenovirus 1774 Ad-RFP-iCre Ad-RFP-2A-Cre

$475.00
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Product Details

Promoter
CMV (ubiquitous)
Reporter
RFP
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.