mouse TOP1 Over-expression AAV
This is an AAV expressing Mouse Top1.
AAV-274812
AAV-m-TOP1
Ready-to-use AAV expressing Mouse Top1 (topoisomerase (DNA) I). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AI467334; D130064I21Rik; Top-1
Description (Vector)
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Gene ID
21969
Gene Name (Vector)
topoisomerase (DNA) I
Gene Symbol
Top1
ORF Size (aa)
2304
ORF Size (bp)
2304 bp
RefSeq ID
NM_009408
RefSeq Synonyms
NM_009408, BC172687, BC156379,
Species
mouse
UniGene ID
Mm.217233
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.