human UPF2 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human UPF2.
shADV-227224
Ad-h-UPF2-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human UPF2 (UPF2 regulator of nonsense transcripts homolog (yeast)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
FRS2/UPF2/LEMD3 fusion; HUPF2; LEMD3/UPF2 fusion; nonsense mRNA reducing factor 2; regulator of nonsense transcripts 2; RENT2; smg-3; smg-3 homolog, nonsense mediated mRNA decay factor; UPF2 regulator of nonsense transcripts homolog; up-frameshift suppressor 2 homolog; yeast Upf2p homolog
Description (eCommerce)
Regulator of nonsense transcripts 2 (RENT2), also known as UPF2, is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). RENT2 is recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B, RENT2 stimulates both ATPase and RNA helicase activities of UPF1. RENT2 binds spliced mRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HAU5].
Description (Vector)
UPF2 encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.
Gene ID
26019
Gene Name (eCommerce)
UPF2, regulator of nonsense mediated mRNA decay
Gene Name (Vector)
UPF2 regulator of nonsense transcripts homolog (yeast)
Gene Symbol
UPF2
HGNC ID
HGNC:17854
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3819
ORF Size (bp)
3819 bp
Protein Name (eCommerce)
Regulator of nonsense transcripts 2
RefSeq ID
NM_015542
RefSeq Synonyms
NM_080599, NM_015542, BC115737, BC114964,
Research Areas
Gene Expression,RNA Binding,RNA Processing,RNA Transport,Transport
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
Regulator of nonsense transcripts 2 (RENT2), also known as UPF2, is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). RENT2 is recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B, RENT2 stimulates both ATPase and RNA helicase activities of UPF1. RENT2 binds spliced mRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HAU5].
UniGene ID
Hs.610110
UniProt ID (eCommerce)
Q9HAU5
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.