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mouse PPP1R12A shRNA silencing AAV

Name 
AAV-m-PPP1R12A-shRNA
Cat No 
shAAV-269188
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Ppp1r12a.

shAAV-269188
AAV-m-PPP1R12A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ppp1r12a (protein phosphatase 1, regulatory (inhibitor) subunit 12A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1200015F06Rik; 5730577I22Rik; AA792106; AV099298; D10Ertd625e; Mypt1
Description (Vector)
Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Gene ID
17931
Gene Name (Vector)
protein phosphatase 1, regulatory (inhibitor) subunit 12A
Gene Symbol
Ppp1r12a
ORF Size (aa)
3090
ORF Size (bp)
3090 bp
RefSeq ID
NM_027892
RefSeq Synonyms
NM_027892, BC137630, BC125381,
Species
mouse
UniGene ID
Mm.476797

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.